Carbapenemases in Enterobacteriaceae: Detection and Antimicrobial Therapy
نویسندگان
چکیده
منابع مشابه
Laboratory detection of Enterobacteriaceae that produce carbapenemases.
A study was designed to evaluate the modified Hodge test (MHT), Mastdiscs ID inhibitor combination disks (MDI), Rosco Diagnostica Neo-Sensitabs (RDS), metallo-β-lactamase (MBL) Etest, and in-house multiplex PCR for the detection of well-characterized carbapenemase-producing Enterobacteriaceae. One hundred forty-two nonrepeat clinical isolates of carbapenemase-producing Enterobacteriaceae (inclu...
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A commercial multiplex PCR (hyplex SuperBug ID) was tested with a collection of 132 clinical Enterobacteriaceae strains producing different carbapenemases. The sensitivity for the detection of KPC-, VIM-, NDM-, and OXA-48-encoding genes was 100%, whereas two IMP variants were missed.
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The most important mechanism of carbapenem resistance in Enterobacteriaceae is the production of carbapenemases, although resistance can also result from the synergistic activity between AmpC-type or (to a lesser extent) extended-spectrum beta-lactamases combined with decreased outer membrane permeability. Three major molecular classes of carbapenemases are recognized: A, B and D. Classes A and...
متن کاملEvaluation of Etest® strips for detection of KPC and metallo-carbapenemases in Enterobacteriaceae.
The performance of Etest KPC and MBL strips (bioMérieux) was evaluated as compared to other phenotypic tests for detecting carbapenemases of the KPC-type and metallo-β-lactamases, respectively, on 133 well-characterized enterobacterial isolates. KPC and meropenem-containing MP/MPI Etest had high sensitivity (>92 %) and specificity (>97 %).
متن کاملprimer evaluation for pcr and its application for detection of carbapenemases in enterobacteriaceae
objectives the objective of this study was to develop the primers for single and/or multiplex pcr amplification assays for simultaneous identification of class a, class b, and class d carbapenem hydrolyzing β-lactamases in enterobacteriaceae and then to evaluate their efficiency. conclusions we have reported here a reliable method, composed of single and multiplex pcr assays, for screening all ...
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ژورنال
عنوان ژورنال: Frontiers in Microbiology
سال: 2019
ISSN: 1664-302X
DOI: 10.3389/fmicb.2019.01823